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eCLIP-seq for Beginners: A Step-by-Step Guide

Submitted by Profacgen on Wed, 09/20/2023 - 23:14

What is CLIP-seq?

Enhanced CLIP-seq (eCLIP-seq) is a method for identifying RNA-binding protein (RBP) binding sites in the transcriptome. It is an improvement on the traditional CLIP-seq method that improves the assay's sensitivity and specificity.

UV irradiation is used to crosslink RNA-binding proteins to their RNA targets in CLIP-seq. The RNA is then fragmented and immunoprecipitated with RBP-specific antibodies. The RBP binding sites can then be identified by sequencing the RNA fragments that were co-immunoprecipitated with the RBP.

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