Profacgen, a leading provider of protein products and protein-related services for life sciences, today announced the enhancement of its fermentation process development solutions to meet the evolving market demands and research needs.
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What is CLIP-seq?
Enhanced CLIP-seq (eCLIP-seq) is a method for identifying RNA-binding protein (RBP) binding sites in the transcriptome. It is an improvement on the traditional CLIP-seq method that improves the assay's sensitivity and specificity.
UV irradiation is used to crosslink RNA-binding proteins to their RNA targets in CLIP-seq. The RNA is then fragmented and immunoprecipitated with RBP-specific antibodies. The RBP binding sites can then be identified by sequencing the RNA fragments that were co-immunoprecipitated with the RBP.