Publisher's Platform: What You Need to Know About Botulism . acterial development is repressed by cooling beneath 4°C, warming above 121°C and high water movement or acidity. And albeit the poison is obliterated by warming it at 85°C for somewhere around five minutes, the spores framed by the microorganisms are not inactivated until the food is warmed to 121°C under high tension for no less than twenty minutes. Publisher's Platform